Regulatory

Part:BBa_K3552017:Design

Designed by: Liu Xibei   Group: iGEM20_LINKS_China   (2020-10-25)


Ptac-SarJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our new ptac promoter consists of 7 components, the reverse lac terminator, lacI, RBS, lac promoter and normal ptac promoter, lacO optimized and SarJ. The new promoter comparing to the BBa_K180000 found in 2009 has a much larger gradient of changing when comparing to the concentration of IPTG. In our experiment, we add different concentration of IPTG to constant promoter and received the value of florescence. We compared the value to the negative control and acquire the stable points to compare the data from the old ptac promoter.



Source

artificially synthesized

References